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R&D Systems c human β glucuronidase enzyme
C Human β Glucuronidase Enzyme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human hpse  (R&D Systems)
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R&D Systems recombinant human hpse
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Recombinant Human Hpse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard recombinant human β galactosidase 1 rhglb1  (R&D Systems)
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R&D Systems standard recombinant human β galactosidase 1 rhglb1
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Standard Recombinant Human β Galactosidase 1 Rhglb1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard recombinant human β galactosidase 1 rhglb1/product/R&D Systems
Average 94 stars, based on 1 article reviews
standard recombinant human β galactosidase 1 rhglb1 - by Bioz Stars, 2026-06
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beta glucuronidase  (R&D Systems)
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R&D Systems beta glucuronidase
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Beta Glucuronidase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/beta glucuronidase/product/R&D Systems
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beta glucuronidase - by Bioz Stars, 2026-06
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heparinase  (R&D Systems)
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R&D Systems heparinase
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Heparinase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heparinase/product/R&D Systems
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heparinase - by Bioz Stars, 2026-06
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recombinant human active heparanase  (R&D Systems)
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R&D Systems recombinant human active heparanase
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Recombinant Human Active Heparanase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human active heparanase - by Bioz Stars, 2026-06
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idua protein  (R&D Systems)
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R&D Systems idua protein
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Idua Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/idua protein/product/R&D Systems
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gh carnoy  (R&D Systems)
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R&D Systems gh carnoy
PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph <t>HPSE</t> (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.
Gh Carnoy, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gh carnoy/product/R&D Systems
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PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph HPSE (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Journal: Infection and Immunity

Article Title: Elevated vaginal heparan sulfate correlates with impaired neutrophil killing of Candida albicans in women with vulvovaginal candidiasis

doi: 10.1128/iai.00709-25

Figure Lengend Snippet: PMN antifungal activity in VCM spiked with HS or treated with HPSEs. Vaginal swab samples were collected from three groups: women with RVVC experiencing a symptomatic acute episode of VVC (symptomatic group, n = 31), those in asymptomatic remission (asymptomatic group, n = 14), and healthy women without a history of frequent VVC (control group, n = 16). The data sets included an additional five follow-up swabs collected from women in the symptomatic and asymptomatic groups. Detached swab tips were suspended individually in 1 mL RPMI 1640 medium to release vaginal secretions and cellular content. Supernatants were sterile-filtered as VCM. PMNs (5 × 10 5 ) isolated from peripheral blood of healthy volunteers were incubated with C. albicans cells (1 × 10 5 ) in 100 µL of VCM or RPMI medium for 3 h at 37°C with 5% CO 2 . Controls consisted of C. albicans cultured alone in VCM or RPMI medium and were used to calculate % killing. ( A ) VCM samples with normal killing activity (non-inhibitory VCM, gray bar) were spiked with purified porcine HS (500 µg/mL) and reevaluated for PMN killing activity (blue bar). Spiked non-inhibitory VCM was then pretreated with r Ph HPSE (3 ng/mL) and reevaluated for PMN killing activity (red bar). ( B ) VCM samples with low killing activity (inhibitory VCM, solid blue bar) were pretreated with rhHPSE (4 µg/mL, red-dotted bar) or r Ph HPSE (3 ng/mL, red-striped bar) and reevaluated for PMN killing activity. After coculture, viable C. albicans cells were enumerated by quantitative plate counts. Data were analyzed using the one-way ANOVA and Bonferroni’s post-test. Bar heights and error bars represent group means ± SEM for % killing values across independent replicates of unique VCM samples. The graphs represent cumulative data from three to four experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Article Snippet: To degrade HS, symptomatic (inhibitory) VCM was pretreated with either recombinant human HPSE (rhHPSE, 4 μg/mL, R&D Systems) or recombinant Pedobacter heparinus heparinase III (r Ph HPSE, 3 ng/mL, R&D Systems) for 1 h at 37°C.

Techniques: Activity Assay, Control, Sterility, Isolation, Incubation, Cell Culture, Purification